Digesting lambda prep

Mario Pineda pinema00 at dons.ac.usfca.edu
Fri May 16 05:56:26 EST 1997


I have done various phage preps in an EMBL3A Library and have yet to be
able to cut my insert out of the phage.  I am digesting with EcoRI and
have tried taking up the prep only in water, overloading the enzyme.  I
read that there may be some problem with using the agar/top agarose
plates.  DOes anyone have any ideas?  Could really use some help.
	Thanks,	
	Mario Javier Pineda



More information about the Methods mailing list