ELISA for Hisx6 proteins

[nozmw at non1.med.nottingham.ac.uk]

Thomas Cameron <cameron at risotto.mit.edu> wrote:

>Has anybody ever set up a ELISA using either Hisx6 tagged proteins or
>S-peptide to S-protein affinity?

>I think I remember seeing an ELISA plate sold by SIGMA that specifically
>binds HIS-tagged proteins, but I can't find the info anymore.

>-- 
>Thomas Cameron
Ni-NTA -HRP or Ni-NTA-Hrp conjugates are also available from Qiagen
for detection of hexahistidine labelled proteins. If anyone has any
experience of these in an ELISA I would be grateful for any
details/tips/teething problems.
Thanks
Michele Writer