Shearing DNA by heat - a question
Paul N Hengen
pnh at ncifcrf.gov
Mon May 19 11:33:14 EST 1997
Susan Hogarth (sjhogart at unity.ncsu.edu) wrote:
> I lost the post, but someone recently described shearing DNA by
> autoclaving or boiling. How long (and at what temp) do you have to let
> it renature? Does it renature "perfectly"?
and Daniel Kim (dkim at nmsu.edu) wrote:
| WRT list of methods to shear DNA:
| One method listed was nebulization.
Can someone tell me what this means?
What kind of device is used for this?
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