How to dissolve sulfathiazole

Peter Wang plw at mrc-lmb.cam.ac.uk
Tue May 20 14:37:23 EST 1997


Can anyone tell me what to dissolve sulfathiazole in and what the stock 
concentration and typical working concentrations for selection are?  I 
want to check the sulfonamide resistance gene in plasmid RSF1010 carried 
in E. coli.  Are there any problems with using sulfonamide resistance as 
a selectable marker for plasmids?  Thanks for any help.

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Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402104  (international calls +44-1223-402104)
Fax (01223) 402140  (     "          "   +44-1223-402140)
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