digesting pcr products

Paul Rudnick rudnick at flash.net
Tue May 20 01:34:07 EST 1997


Hello-

I have been repeatedly trying to clone a pcr product generated from
primers containg engineered restriction sites.  I am aware that some
enzymes require more than a few bases downstream to properly cut,
however I did check a chart for cutting the ends of fragments and these
enzymes should have sufficient room.  The product was generated with pfu
and the enzymes are HindIII and HincII.  Any ideas why the product won't
clone?

Thank in advance!

Paul



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