T7, T3 and SP6 transcription

brett brett at BORCIM.WUSTL.EDU
Wed May 21 09:09:26 EST 1997

>Hi Folks,
>Hopefully an easy question.
>Does one need extra bases before the start of the T7, T3 or SP6 specific
>promoter sequences to get good transcription ie can I PCR using a T7
>primer then do a transcript from that or am I better using an initial
>PCR primer upstream of the T7 promoter sequence?
>Many thanks
>The problem with being on the cutting edge is that you occasionally get 
>sliced from time to time....
>Duncan Clark
>DNAmp Ltd.
>TEl/FAX 01252376288

yes. this will work fine.
Brett Lindenbach
Dept Molecular Microbiology
Washington University School of Medicine
St Louis, MO

"One thousand miles an hour, I'm just like anyone.
I want to feel the road of tar beneath the wheel
named extinction" - Pixies

More information about the Methods mailing list