T7, T3 and SP6 transcription

brett brett at BORCIM.WUSTL.EDU
Wed May 21 09:09:26 EST 1997


>Hi Folks,
>
>Hopefully an easy question.
>
>Does one need extra bases before the start of the T7, T3 or SP6 specific
>promoter sequences to get good transcription ie can I PCR using a T7
>primer then do a transcript from that or am I better using an initial
>PCR primer upstream of the T7 promoter sequence?
>
>Many thanks
>
>Duncan  
>-- 
>The problem with being on the cutting edge is that you occasionally get 
>sliced from time to time....
>
>Duncan Clark
>DNAmp Ltd.
>TEl/FAX 01252376288
>http://www.dnamp.com
>http://www.genesys.demon.co.uk

yes. this will work fine.
Brett Lindenbach
Dept Molecular Microbiology
Washington University School of Medicine
St Louis, MO

"One thousand miles an hour, I'm just like anyone.
I want to feel the road of tar beneath the wheel
named extinction" - Pixies




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