T7, T3 and SP6 transcription
brett at BORCIM.WUSTL.EDU
Wed May 21 09:09:26 EST 1997
>Hopefully an easy question.
>Does one need extra bases before the start of the T7, T3 or SP6 specific
>promoter sequences to get good transcription ie can I PCR using a T7
>primer then do a transcript from that or am I better using an initial
>PCR primer upstream of the T7 promoter sequence?
>The problem with being on the cutting edge is that you occasionally get
>sliced from time to time....
yes. this will work fine.
Dept Molecular Microbiology
Washington University School of Medicine
St Louis, MO
"One thousand miles an hour, I'm just like anyone.
I want to feel the road of tar beneath the wheel
named extinction" - Pixies
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