T7, T3 and SP6 transcription

Dr. Duncan Clark duncan at genesys.demon.co.uk
Wed May 21 06:00:33 EST 1997

Hi Folks,

Hopefully an easy question.

Does one need extra bases before the start of the T7, T3 or SP6 specific
promoter sequences to get good transcription ie can I PCR using a T7
primer then do a transcript from that or am I better using an initial
PCR primer upstream of the T7 promoter sequence?

Many thanks

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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