T7, T3 and SP6 transcription
Dr. Duncan Clark
duncan at genesys.demon.co.uk
Wed May 21 06:00:33 EST 1997
Hopefully an easy question.
Does one need extra bases before the start of the T7, T3 or SP6 specific
promoter sequences to get good transcription ie can I PCR using a T7
primer then do a transcript from that or am I better using an initial
PCR primer upstream of the T7 promoter sequence?
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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