Long accurate PCR-help

Bonnie L. Balzer balzer at LELAND.STANFORD.EDU
Wed May 21 16:43:32 EST 1997


For some time I am trying to amplify large fragment from Streptomyces
linear plasmid (16 kb). Apparently I wasn=92t very successful. Though I
changed different parameters, denaturation temp., annealing temp.,
enzyme concentration ( I am using Klentaq LA as well as Elongase from
LTI), added betaine or DMSO, I still get nothing. Did anyone have luck
with large G+C rich template. If someone has any useful suggestion I
will be very grateful.

Kenan Pandza
kpandza at cmgm.stanford.edu



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