yeast plasmid prep protocol?

Donny Wong wong5 at login2.fas.harvard.edu
Thu May 22 23:42:57 EST 1997


hi netters,

does anyone have a good protocol for isolating plasmid DNA from
saccharomyces cerevisiae?  i've had problems using a glass bead protocol
from the redbook and nuclease problems when using a protocol that calls
for zymolyase treatment followed by a wizard mini-prep.  

thanks!
donny wong
wong5 at fas.harvard.edu



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