T4 Pol killed the DNA

Claus Rodemer c.rodemer at dkfz-heidelberg.de
Fri May 23 04:09:30 EST 1997


Hi Netters
I used T4 Pol to make blunt ends (protocol like Maniatis), but as result  I
got a smear all over the lane. It seems that the DNA was randomly cut. What
happened ?  I used new T4 Pol ,it shouldnt be contaminated.

Thanks
Claus
--

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Claus Rodemer  German Cancer Research Center  INF 280
Div.Biophysics of Macromolecules  AG. Gene Regulation and DNA Topology
D-69120 Heidelberg  E-Mail: C.Rodemer at dkfz-heidelberg.de
WWW:http://www.rzuser.uni-heidelberg.de/~crodemer/
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