Q : do common delta(lacZ) constructs also have lacI deletions?
gstuart at uvic.ca
Fri May 23 02:24:30 EST 1997
Does anyone know if the common lacZ deletions, e.g. del(argF-lac)U169,
del(YZA), del(lac-proAB) include the lacI gene?
I am interested in having alpha-complementation being established by
having the host cells provide the omega-LacZ fragment (from
lacZdeltaM15), with the alpha-LacZ fragment being provided from my
incoming phage. The alpha-LacZ fragment is linked to the lacI gene,
which is my target for mutational analysis. I don't want my data
complicated due to interactions of mutated incoming lacI protein
interacting with endogenous, wild-type repressor protein.
Has anyone ever sequenced the lac region in these strains (e.g.,
DH5alpha-MCR)? Is this information available somewhere? While I'm at
it, is the sequence information for lacIq-lacZdeltaM15 also available?
Thanks, Greg :)
Greg Stuart gstuart at uvic.ca
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