T4 Pol killed the DNA

Zophonias O. Jonsson zjons at vetbio.unizh.ch
Fri May 23 09:21:16 EST 1997


In article <5m3msa$svj at mserv1.dl.ac.uk>, Claus Rodemer
<c.rodemer at dkfz-heidelberg.de> wrote:

> Hi Netters
> I used T4 Pol to make blunt ends (protocol like Maniatis), but as result  I
> got a smear all over the lane. It seems that the DNA was randomly cut. What
> happened ?  I used new T4 Pol ,it shouldnt be contaminated.
> 
Hi Claus

   Did you forget the dNTP's ???

Zophonias

_____________________________________________________________________
Zophonias O. Jonsson
Institut fur Veterinarbiochemie               Tel: (41-1)-257-54-75
Universitat Zurich-Irchel                     Fax: (41-1)-362-05-01
Winterthurerstrasse 190
CH-8057 Zurich
Switzerland
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