T4 Pol killed the DNA
PHADMH at leeds.ac.uk
Sat May 24 03:55:34 EST 1997
In article <5m3msa$svj at mserv1.dl.ac.uk>,
Claus Rodemer <c.rodemer at dkfz-heidelberg.de> wrote:
>I used T4 Pol to make blunt ends (protocol like Maniatis), but as result
>got a smear all over the lane. It seems that the DNA was randomly cut.
>happened ? I used new T4 Pol ,it shouldnt be contaminated.
I had the same problem with T4 despite diluting it and using shorter
reaction time. Klenow fragment does the job just fine though.
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