T4 Pol killed the DNA

D. Mchugh PHADMH at leeds.ac.uk
Sat May 24 03:55:34 EST 1997


In article <5m3msa$svj at mserv1.dl.ac.uk>,
   Claus Rodemer <c.rodemer at dkfz-heidelberg.de> wrote:
>Hi Netters
>I used T4 Pol to make blunt ends (protocol like Maniatis), but as result 
 I
>got a smear all over the lane. It seems that the DNA was randomly cut. 
What
>happened ?  I used new T4 Pol ,it shouldnt be contaminated.
>
>Thanks
>Claus

I had the same problem with T4 despite diluting it  and using shorter 
reaction time.  Klenow fragment does the job just fine though.

Damian



More information about the Methods mailing list