T4 Pol killed the DNA
vkraynov at pop.service.ohio-state.edu
Sat May 24 20:45:29 EST 1997
In article <5m3msa$svj at mserv1.dl.ac.uk>, Claus Rodemer <c.rodemer at dkfz-heidelberg.de> wrote:
>I used T4 Pol to make blunt ends (protocol like Maniatis), but as result I
>got a smear all over the lane. It seems that the DNA was randomly cut. What
>happened ? I used new T4 Pol ,it shouldnt be contaminated.
3'->5' exo of T4 is at work !.. Try a different enzyme, or use excess dNTPs.
More information about the Methods