T4 Pol killed the DNA

Vadim Kraynov vkraynov at pop.service.ohio-state.edu
Sat May 24 20:45:29 EST 1997

In article <5m3msa$svj at mserv1.dl.ac.uk>, Claus Rodemer <c.rodemer at dkfz-heidelberg.de> wrote:
>Hi Netters
>I used T4 Pol to make blunt ends (protocol like Maniatis), but as result  I
>got a smear all over the lane. It seems that the DNA was randomly cut. What
>happened ?  I used new T4 Pol ,it shouldnt be contaminated.

3'->5' exo of T4 is at work !..  Try a different enzyme, or use excess dNTPs.

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