Two-hybrid false positives

simonh at bcm.tmc.edu simonh at bcm.tmc.edu
Sun May 25 13:42:25 EST 1997


Dear netters,

Does anybody have a list of most common false positives
in the two-hybrid assay? Using several "baits" as a control
doesn't help always. For a example, when you have related proteins
that may interact with the same target.
Number one in false positives are DNA-binding proteins. I'll appreciate
any additions to this list.

Simon Hoffenberg
Baylor College of Medicine
Houston, TX

-------------------==== Posted via Deja News ====-----------------------
      http://www.dejanews.com/     Search, Read, Post to Usenet



More information about the Methods mailing list