how do I concentrate metaphase chromosomes?

telenius at telenius at
Tue May 27 01:41:11 EST 1997

I need to take 300,000
metaphase chromosomes
and put them in a volume
of 20 microliter. Does
anyone have
protocol/suggestion for
how to concentrate
metaphase chromosomes
in a gentle manner so as
not to damage their
integrity? The need to
remain 'viable' after the
concentration step.
Thank you!

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