Zhong Zhao BioChem
zzhao at lenti.med.umn.edu
Mon May 26 13:08:07 EST 1997
I am trying to insert a 5 kb rat genomic sequence into pXP1 vector, which
contains luciferase gene, pBR322 and some SV40 fragments.
Even I tried many times. I have not got any luck. Few colonies of
transformants appear on the plate but none of them is right. Control study
shows the competent cells are fine.
Since the vector itself is 6.1kb, I wonder how much extra DNA this vector
can hold. If you have experience of inserting big piece of DNA into this
vector, please let me know.
Thank you so much.
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