Ligation with T4 ligase

Estanislao Ramirez eramirez at BITMED.MED.UCHILE.CL
Mon May 26 12:01:47 EST 1997


Dear Everybody:
I've got a problem with a ligation with T4 ligase.  I have purified two
DNA fragments from an agarose gel.  Each of the fragments has sticky
ends (cut with Hind III and Xba I) and therefor should be
complementary.  Is there any way to investigate the efficiency of a
ligation before attempting a transformation?  thanx

Alfredo Ramirez
School of Medicine
Universidad de Chile



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