lipofection for Vero cell line

[Ramon Garcia Escudero]

We are trying to have a efective method for lipofection of E. coli
plasmids and cosmids in Vero cells. The problem is that there are many
commercial lipids for DNA transfer in mammalian cells but results for
Vero cells (green monkey kidney) are not described. Do anyone know  a
efficient method for lipofection of plasmid DNA in this cell line?

Thanks for help


Ramon Garcia Escudero

Centro de Biologia Molecular "Severo Ochoa"
Facultad de Ciencias
Universidad Autonoma de Madrid
Campus de Cantoblanco
28049 MADRID
Spain

Mailto:rgescudero at trasto.cbm.uam.es
Phone: 34 1 3978438
Fax: 34 1 3974799