Ligation with T4 ligase

Els Van Geldre Els.VanGeldre at rug.ac.be
Tue May 27 09:51:08 EST 1997


Hi Alfredo,

Maybe you could do this with PCR, if you know the sequence or part of the 
sequence of your DNA fragments. Other protocols say to run an aliquot of 
your ligation reaction on an agarose gel (but therefore you need to have 
sufficient product). If your fragments completely ligated you schould see 
one band on gel. In the other case you still see both the fragments 
eventually with a third band due to your ligation product.

Els




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