Reply to Fast& Cheap Plasmid Miniprep Kit
R.SKILTON at CGNET.COM
Thu May 29 01:28:26 EST 1997
Bio 101 sell a plasmid purification resin that costs a few cents per prep.
(This is not an advert!) It works fine for digests, but I'm not sure if it's
good enough for sequencing.
To: bionet.molbio.methds-reagnts mail newsgroup
Subject: Re: Reply to Fast& Cheap Plasmid Miniprep Kit
Date: Wednesday, May 28, 1997 11:48PM
Qguo at aol.com wrote:
>I have used many different commercial kit for Plasmid miniprep, usually
>Quagen's plasmid Purification kit worked well, it worked stable, and fast.
>But recently I am using Omega Biotek's miniprep kit, it works very good,
>very good yield as Quagen, it is a little bit fast, one miniprep takes only
>10 mins, average yield is about 30mg, the most important reason for me to
>this kit is that it is much cheap: only $35 for 50 minipreps. I think it is
>good deal. I hope you call them to asking a free sample or visit their Web
>Page: www. Omegabiotek.com. I hope this information can help you at some
>point. Good luck for your experiment.
I don't want to spoil anyone's business ;-) and to re-ignite
"anti-kit" polemics (where are you, Jim?), but why, for crying out
loud, are you guys spending all that time AND money when alkiline
lysis with NH4Ac neutralization gives such a quick and wonderfully
pure miniprep? Yes, it is good enough for automatic sequencing and NO,
it is not good enough for transfection. 18 minis take about 45 - 60
min and you get up to 2 ug plasmid from 1.5 ml of o/n culture. I have
not calculated the cost per miniprep (2 epp tubes are the most
expensive items, I believe), but it surely beats ANY kit.
Victor Levenson MD/PhD
Res Asst Prof
UIC, Dept of Genetics M/C 669
900 S Ashland Ave
Chicago IL 60607
levenson at uic.edu
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