Western blot from native PAGE
white8 at server.uwindsor.ca
Wed May 28 13:23:54 EST 1997
I am doing a native PAGE of my protein solution and
would like to use this gel for a western blot. Are there
any special considerations to keep in mind when doing a
blot from a native gel rather than a gel from an SDS-PAGE?
For example is it better to use a transfer buffer that does
contain SDS or one that just contains methanol and buffer?
Thanks in advance for any help.
Department of Chemistry and Biochemistry
University of Windsor
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