Western blot from native PAGE

White Stephanie white8 at server.uwindsor.ca
Wed May 28 13:23:54 EST 1997


I am doing a native PAGE of my protein solution and 
would like to use this gel for a western blot.  Are there
any special considerations to keep in mind when doing a
blot from a native gel rather than a gel from an SDS-PAGE?
For example is it better to use a transfer buffer that does
contain SDS or one that just contains methanol and buffer?

Thanks in advance for any help.

Stephanie White
Department of Chemistry and Biochemistry
University of Windsor
Windsor, Ontario



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