best method to lyse bacteria for recombinant protein purification

Malathi Hari mhari at expert.cc.purdue.edu
Wed May 28 17:24:17 EST 1997


 What is in your opinion and experience the best method to lyse bacteria 
to purify soluble recombinant protein.

Freeze thaw and Sonication seem to leave behind a large amount of unlysed 
bacteria thus increasing loss of preciuos protein.

Thanks

Malathi




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