look for a plasmid with a different ori of replication

Cheng Luo cheng.luo at plantphys.umu.se
Thu May 29 03:14:09 EST 1997


Dear netters,

I am cloning 2 genes with separate different plasmids, I would like to
have a plasmid with an ori of replication different from ColE1, such as,
with p15a origin of replication, and with a resistence gene rather than
Tet and Ap, fit with the NcoI and BamHI cloning in the downstream of T7
promoter and rbs. Please let me know if you happen to have or the
information.  thanks!  Cheng



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