vectors with poly(A)

[ijiwaru at wheel.dcn.davis.ca.us]

In article <97May29.041752edt.27779 at firewall.smh.toronto.on.ca>,
VASCULAR at SMH.TORONTO.ON.CA (Lab Vascular) wrote:

> Hi !  
> Does anyone know of a good vector which gives a synthetic poly-A tail
> to the RNA transcribed from it in vitro, pls ?
> I mean, besides, Promega's pSP64 Poly(A) <-- the tail is too short,
> and my insert has EcoRI sites (so I can't linearize ! HELP !! ).
> 
> Thanks !
> 
> Patrick F.H. Lai
> PhD student, U of Toronto
> 97MAY29

Patrick,

have you considered using poly-A polymerase and ATP to tail your
transcript?  I made a modification of pSP64 poly-A but it has the same
sites and same A-tail length (as far as I could tell) so probably wouldn't
do you much good.  You could also try not linearizing or linearizing
further downstream.

Good Luck.

Lyle Najita
Plant Pathology
University of California - Davis