5'end labeling

Nick Jacobsen jacobsen at cf.ac.uk
Fri May 30 08:37:58 EST 1997


Hi,
   For microsatellite PCR we end label with 33P (1000-3000Ci/mmol, 
10mCi/ml). We label 200pM primer using 3µl of the above isotope in a 
reaction. This gives efficient end labelling for about 50 PCR reactions. 
You should be able to use much less 32P.




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