Activity restored by mixing recombinant domains?

Dennis Benjamin ocms0001 at
Mon Nov 3 08:45:18 EST 1997

Lloyd Graham (lloyd.graham at wrote:

: I'm looking for any precedents where the domains of a monofunctional
: enzyme have been expressed separately as inactive proteins, but when mixed
: together they associate to form a complex with significant enzymatic
: activity. 

Markby et. al., Science 262 p1895 (1993), separately expressed 2 halves of
a heterotrimeric G protein alpha chain; GTPase activity was regained
upon mixing the two domains.


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