Activity restored by mixing recombinant domains?
ocms0001 at ermine.ox.ac.uk
Mon Nov 3 08:45:18 EST 1997
Lloyd Graham (lloyd.graham at molsci.csiro.au) wrote:
: I'm looking for any precedents where the domains of a monofunctional
: enzyme have been expressed separately as inactive proteins, but when mixed
: together they associate to form a complex with significant enzymatic
Markby et. al., Science 262 p1895 (1993), separately expressed 2 halves of
a heterotrimeric G protein alpha chain; GTPase activity was regained
upon mixing the two domains.
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