RNA electrophoresis!!

Robert W. St. G. Fisher, IV fisher at radonc.unc.edu
Mon Nov 3 17:00:58 EST 1997


what i've been doing recently is staining the membrane
(Boehringer-Mannheim, their nylon stuff) with methylene blue in an
acetate buffer after the transfer.  You destain in 5% acetic acid.
Works great, although it is tough to get the blue all the way out.
Someone else in our lab read in Biotechniqes that you can completely
destain by using a SSPE/SDS buffer.

Robert Fisher
fisher at radonc.unc.edu




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