RNA electrophoresis!!

gj at nospampobox4.stanford.edu gj at nospampobox4.stanford.edu
Mon Nov 3 20:53:37 EST 1997


Hi iuse methylene blue also but have not problem destaining. the trick
is not to stain to long. the methylene blue is in .3M NaOAC ph 5.0 put
this on membrane for five to ten seconds then wash extesively with
water.  The stain won't interfer in hyb and washes off in prehyb
solution. To remove the stain just use a little SDS and heat to 50 
stain if you don't like blue hyb solutions.



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