flourescence quenching

R.D. Haigh rxh at leicester.ac.uk
Mon Nov 3 13:38:09 EST 1997

 kreider at crab.rutgers.edu (Barbara Kreider) 


  Does anyone know of a compound to add to mounting medium to reduce
  fluorescent quenching? Vectashield is effective, but too expensive. 

Hi Barbara, 
I have always used glycerol-PBS containing 1 mg/ml p-phenylenediamine
as my mounting solution - it seems to work well with both FITC and
TRITC. Its in the Sigma catalogue at only =A34.50 for 50g so its worth a
try whatever you are using. Store at -20C in the dark. 


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