Fluorescent quenching

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Mon Nov 3 13:25:58 EST 1997


Hi Barbara.

If you're looking for an antifade agent, try this: 

It's 5% n-propyl gallate (e.g. from Sigma) in Glycerol, one drop 
added on the sample before placing the coverslip.

Ref: Science (1982) 217, 1252-5

Good luck!
Wolfgang

> Does anyone know of a compound to add to mounting medium to reduce
> fluorescent quenching? Vectashield is effective, but too expensive.
> 
> Thanks.
> Barbara Q. Kreider, Ph.D.
> Assistant Professor of Biology
> Rutgers, the State University
> Room 202 Science Building
> 315 Penn Street
> Camden NJ 08102-1411
> Phone:	609/225-6553
>  609/225-6335
> FAX:	609/225-6312
> 
> "There can be only one."
> 
> 
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Wolfgang Schechinger         
University of Tuebingen
email: wgschech at med.uni-tuebingen.de
http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
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