low rna concentration
joseph at rockefeller.univ-lyon1.fr
Mon Nov 3 12:54:40 EST 1997
I've got a problem using the SV RNA isolation kit from promega on (very
!) little amount of tissue; the total concentration of RNA at the end of
the isolation is very low (about 2 microg in 100 microl of water, i need
quite a large volume to read DO at 260 nm) :
- is ther another way to determinate the RNA concentration, which would
not need large amount of RNA ?
- can i make the last elution (with water) in a volume < 100 microl ?
- is there any possibility of drying the RNA (alcohol precipitation ?
like in the clasical phenol/chloroforme exctraction) ? after the elution
thank you for replying
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