RNA electrophoresis
Patrick HJ Falckh
p.falckh at rmit.edu.au
Tue Nov 4 19:00:19 EST 1997
Hi y'all,
I also add Ethidium bromide to my RNA samples before I heat shock them.
The major difference is that when the samples are heat shocked with EtBr
then the resultant bands are approx. 10X brighter than when you stain
the gel itself or when you add the EtBr after heat shocking. I've never
had any problems with this method (initially came from Focus 10.1, I
think) and you will use considerably less EtBr, hence lowere incidence
of accidents etc.
Regards
--
Patrick HJ Falckh PhD # #
Key Centre for Applied & Nutritional Toxicology # # # # #
RMIT - City Campus # Q Q #
GPO Box 2467V, Melbourne Vic 3001 # o #
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Telephone : 03 9660 3136 ##<->##
Fax No. : 03 9663 6087 # #
Email : p.falckh at rmit.edu.au # # # #
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"No matter how tall your grandfather # \/ #
was, you have to do your own growing" # /\ #
ooO Ooo
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