Genomic Gap Cloning

Gene Cloning sa62503 at odin.mdacc.tmc.edu
Tue Nov 4 19:04:56 EST 1997


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Gene Cloning, a new company in the field of genomics, offers the 
service to close the gaps between contigs for genomic research. 
With a powerful new technology that allows fast gap closing, 
Gene Cloning can save your time and cost in your microbial genome 
projects. Should you need more information, please contact us. 
We are looking forward to working for you.
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GENE CLONING OFFERS:
CONSTRUCT FRACTIONATED cDNA LIBRARY.
SCREENING GENE LIBRARY.
SUBTRACT LIBRARY.
SUB-CLONING.
EXPRESSION GENE IN VITRO.
SOUTHERN, NORTHERN, WESTERN BLOT.
DNA TESTING.
IN SITU HYBRIDIZATION.
IMMUNOCHEMITOHISTORY   &
PREPARING SOLUTIONS.(call today, deliver to lab. tomorrow)

GENE CLONING CONSULTED BY:
MOLECULAR BIOLOGISTS: Dr. L. Gan,  Dr. L. Li,  & Dr. Z. Wang.

2990 Richmond, Suite 330
Houston, TX 77098
Tel: (713) 522-5550
Fax: (713) 522-7447
Email: clonclon at hotmail.com


GENE CLONING OFFERS:

CUSTOM LIBRARY CONSTRUCTION
* ZAP expression & ZAP two Hybrids System vector 
   available, which contains prokaryotic & eukaryotic 
   promoters,unidirectional,specifically, the full length 
   cDNA can be cut out with restriction enzyme.
* Any selection of vectors.
* Any choice of mRNA priming method for cDNA 
   libraries.
* Quality control data provided with each library.
* Complete protocols and appropriate host strain(s) 
   sent with all custom libraries.


cDNA Library Vector	Cat #	Price $
Zap expression	     GC2001	4800
Zap II	             GC2002	4800
Lambda gt10	     GC2003	4000
Lambda gt11          GC2004	4000
Your choice	     GC200X	Negotiable

Genomic Library		
EMBL 3	             GC2011	4600
EMBL 4               GC2012	4600
EMBL3Sp 6/T7	     GC2013	4600
Cosmid pw 15	     GC2014	5100
Your choice	     GC201Y	Negotiable
PolyA RNA prep.	     GC2031	1500
Genomic DNA prep.    GC2032	1000

SUB-CLONING
* Any selection of vectors.
* Insertion region will be checked by sequencing.
* Insert size between 250-1500 BP are available.
   Insert size <250 or >1500 BP, the price are 
   negotiable.

Transcription Vector
	                End     Cat #	Price$
Bluescript	        sticky	GC1013	2000
Bluescript	        blunt 	GC1014	3000
pGEM- 3,5,7,9,11,13	sticky	GC1017	2000
pGEM- 3,5,7,9,11,13	blunt	GC1018	3000
pSp- 64,65,64poly(A)	sticky	GC1021	2000
pSp- 64,65,64poly(A)	blunt	GC1022	3000

Expression vector			
pGEMEX-1,2	  sticky	GC1025	2300
pGEMEX-1,2	   blunt	GC1026	3300
pSG5	          sticky	GC1027	2300
pSG5	           blunt	GC1028	3600
pSV-beta-Galactosidasesticky	GC1029	2300
pSV-beta-Galactosidase blunt	GC1030	3600
			
Specialized Vector  End	       Cat #	Price$
pALTER-1          sticky      GC1031	2300
pALTER-1	  blunt	      GC1032	3600
pGEM-Luc	  sticky      GC1033	2300
pGEM-Luc	  blunt	      GC1034	3600
pGEM-T	          sticky      GC1035	2300
pGEM-T	          blunt	      GC1036	3600

Reporter Vector			
pGLE2-system	sticky	      GC1037	2300
pGLE2-system	blunt	      GC1038	3600
 

OTHER SERVICES
 
	                   CAT #	PRICE$
DNA EXTRACTION
FROM SLIDE(QUALITY 
FOR PCR)	          GC1041	500
DNA TESTING		
 DOT BLOT(5-6 PROBES)     GC1045	500
 
RFLP   ( 3 PROBES )	
                          GC1046	600
    		
		
IN SITU HYBRIDIZATION
(FOR 20 SILDES.)	
                          GC1043	
                                        2000
GENE EXPRESSION
IN VITRO	          GC1040	NEGOTIABLE
SCREENING GENE
LIBRARY	                  GC1044	NEGOTIABLE

DNA SEQUENCING	          GC1040	NEGOTIABLE
GENE SPECIAL:
FRACTIONATED(10 frctions)    
cDNA library              GC1050      $ 20,000

Additional special:
Special preperation                  GC9999           negotiable
Coming to lab. service               GC0922           negotiable
Trouble shooting                     GC0911           negotiable        

Please call (713) 522-5550
       Fax  (713) 522-7447
       Email clonclon at hotmail.com
For more information




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