Q: Do DNA polymerases hop over gaps?

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Tue Nov 4 12:45:32 EST 1997

Salvete, molbio godesses and gods!

Please imagine two primers with a small gap (1 to 4 nucleotides 
long) between them annealed to a piece of DNA. Will polymerases stop 
at this gap  waiting for nucleotides to come and do e.g. some 
proofreading while hanging around ? 
or will they just run over the gap, leaving it unfixed?

Any ideas, opinions, knowledge?

I Appreciate all input.

usual disclaimers apply
Wolfgang Schechinger         
University of Tuebingen
email: wgschech at med.uni-tuebingen.de
!Junk mail is *not* appreciated!
SPAMs will be answered automatically by sending my 128MByte virtual memory file.
This is *NO* joke.

More information about the Methods mailing list