Q: Two-hybrid library amplification

Bin Zheng bzheng at UCSD.EDU
Tue Nov 4 12:48:40 EST 1997


Hi, there,

Does anybody there have a good protocol for the amplification of two hybrid
cDNA lib., except the one in CLONTECH's manual?  CLONTECH recommend the
solid plate method instead of liquid culture, but I heard that somebody use
liquid cluture method and get good result. Any suggestion will be highly
appreicated.

Bin Zheng





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