Ligation question, quite simple

Wolfgang Kemmner kemmnerw at rrkchir.mdc-berlin.de
Wed Nov 5 09:19:35 EST 1997


Hi all, 

we are doing (first time!) some ligation reactions. Now the manual says:
after 25-fold overdigestion, 95% of the DNA fragments can be ligated.
What do they mean: 25 fold more restriction enzyme, or what ?

Thanks for simple answers
Wolf

   




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