expression with BL21(DE3)

Barbara Moffatt moffatt at SCIBORG.UWATERLOO.CA
Wed Nov 5 14:19:29 EST 1997


Hello:

I have a colleague that wishes to transform a plasmid library created from
that Stratagene lambda zap (unizap) vector into BL21(DE3) for expression
from the T7 promoter. Can anyone comment on the expression levels from a
non pET vector, using a BL21(DE3) host? Novagen says they don't guarantee
it and I only have experience using pET vectors with this host.
Thanks in advance for your assistance.

Barb Moffatt
moffatt at sciborg.uwaterloo.ca





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