Internal Standard for Northern

elader at ambion.com elader at ambion.com
Wed Nov 5 12:02:46 EST 1997


In article <34512732.238CDC58 at facstaff.wisc.edu>,
  pemozdzi at facstaff.wisc.edu wrote:
>
> What are some of the disadvantages of using an 18S or 28S probe as an
> internal standard for a Northern? Are they better than GAPDH?

Someone mentioned the high signal from rRNA - yup. Make the probe cooler!
If you use random primed probes, add cold dCTP at 200X the molarity of
the 32P dCTP. Hmmn,,, I wonder if this works. Probably will.

Eric

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