expression with BL21(DE3)

Armin Sepp, PhD asepp at rpms.ac.uk
Thu Nov 6 03:30:38 EST 1997


Dear Barb,
I have successfully used BL21(DE3)pLysS (Novagen) for tac promoter
driven expression, the presence of T7 RNApol after IPTG induction does
not seem to matter. If your colleague wants to transform the library
into BL21-s then there might be a problem with transformation efficiency
which can be significantly (ca 100 times in my hands) worse than this of
conventional cloning strains.

Good luck
	armin sepp
	a.sepp at rpms.ac.uk



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