internal standards for northerns

deborah.hailstones at smtpgwy.agric.nsw.gov.au deborah.hailstones at smtpgwy.agric.nsw.gov.au
Thu Nov 6 17:26:40 EST 1997


     In article <34512732.238CDC58 at facstaff.wisc.edu>,
     pemozdzi at facstaff.wisc.edu wrote:
     >
     > What are some of the disadvantages of using an 18S or 28S probe as 
     an > internal standard for a Northern? Are they better than GAPDH?
     
     I have routinely used 18S for standardisation, and to overcome the 
     problem of signal strength and also to ensure that the probe isn't 
     limiting (which would totally destroy any chance of quantitating using 
     the probe...), i always add a bucket load of cold 18S oligo to the 
     hyb.... ie add end labelled and cold together, in say a 1/1000 
     ratio....
     
     deb





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