RNase-inhibiting agents

Ralf Oltmanns Biologist at SpyRing.com
Thu Nov 6 06:07:10 EST 1997


I am trying to extract mRNA from soil bacteria via direct lysis.
Therefor I need a very potent RNase-inhibiting agent.
I used GITC (guanidin-isothiocyanate) and DEPC (diethyl-pyrocarbonate)
in different protocols with not really satisfactory results, whereas
they really work fine with pure cultures.

I would be grateful for any advice.

Thanx
Ralf

(Please reply by mail, too, 'cos' I am not following this group
regularly. Thanx again)

--
### We, the Willing,
### led by the Ignorant,
### do the Impossible for the Ungrateful





More information about the Methods mailing list