restriciton enzyme site

Marcel Tanudji mtanudji at cyllene.uwa.edu.au
Fri Nov 7 02:02:12 EST 1997


hi all,

i have a question.  the restricion site for sma I is that of

5'cccggg3'
3'gggccc5'

now if you have the sequence 

5'gggccc3'
3'cccggg5'

would the enzyme still recognise it and does it matter if its on a linear
or circular DNA.

  -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
  =  Marcel Tanudji 						        =	
  =  Dept Biochemistry,  University of Western Australia	        =
  =  Perth, Australia	[email:mtanudji at cyllene.uwa.edu.au]             =
  =								        =     
  -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-




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