anti-DIG FAb-HRP and nitrocellulose?

Michael J. Prigge prigge at darkwing.uoregon.edu
Fri Nov 7 01:34:02 EST 1997


Hi, 
I have been using BMB's Genius system for Southern blotting, although 
I switched to using the HRP (or POD) conjugated antibody. Now I am 
screening a phage cDNA library, and the manual says not to use 
nitrocellulose with their normal chemiluminescent detection kit (which 
would use the Alkaline Phosphotase conjugated antibody). I was wondering 
if anyone knew whether the membrane restriction would be specific to the 
AP antibody or if the HRP antibody would similarily be affected.

Thanks
Mike

-- 
Michael Prigge                          Phone: (541) 346-4256
Institute of Molecular Biology            Fax: (541) 346-5891
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