PCR based labeling, library Screen

Jutta Steinkoetter jutta+ at andrew.cmu.edu
Thu Nov 6 19:29:30 EST 1997


Hi everyone

I am looking for a working protocol for a 32P labeling reaction of DNA
via PCR so I can use the product as a probe for Screening genomic and
cDNA libraries.

The DNA has a very high GC content, so I would like to use alpha32P-dCTP. 
Hot denaturation temp. and short and stringent annealing conditions are
what I use in normal PCR reactions. 

Who has experience in this?

Jutta Steinkoetter


Jutta+ at andrew.cmu.edu

Mellon Institute
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Pittsburgh 15213 PA

phone 412 268 3398






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