Sequencing Gels Not Sticking to Whatmann

z z-suldan at ski.mskcc.no.spam.org
Fri Nov 7 07:41:08 EST 1997


In article <kirkpat-0611971756420001 at gietz.hgen.umanitoba.ca>,
kirkpat at cc.?umanitoba?.ca (Rob Kirkpatrick) wrote:

>    One of the people in our lab is having a unique problem.  After running
> her sequencing gels she places the Whatman paper on the gel to remove it
> from the front plate and then disaster strikes.  The gel does not want to
> come off the glass plate and rips to shreds.  This has happened twice now
> and I can't figure it out.  The backplate is "Sigma Coated" so we can't
> really apply this to the front plate (or can we?).  We haven't changed the
> Whatman or anything else that I can think of...  Has anyone ever
> encountered this before???
> 
> Rob


I had a similar problem a year back. In my case, our technician finally
figured out that I was running my gels too hot. For the 35 well gels, I
now run at 35watts and for the 60 well, 55 watts.

Good luck,
Zal



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