Hi everyone, I have very high GC rich DNA to amplify (300 to 3000 bp). Who knows wich Taq Polymerase works best? Which conditions? (Ifound 5% DMSO to be essential, Annealing with 15 sec 58C) ! What else? Jutta Steinkoetter Jutta+ at andrew.cmu.edu Mellon Institute 4400 Fifth Avenue Pittsburgh 15213 PA phone 412 268 3398