PCR witg high GC

Jutta Steinkoetter jutta+ at andrew.cmu.edu
Fri Nov 7 10:06:47 EST 1997


Hi everyone,

I have very high GC rich DNA to amplify (300 to 3000 bp).
 
Who knows wich Taq Polymerase works best?

Which conditions? (Ifound 5% DMSO to be essential, Annealing with 15 sec 58C) !

What else?

Jutta Steinkoetter


Jutta+ at andrew.cmu.edu

Mellon Institute
4400 Fifth Avenue
Pittsburgh 15213 PA

phone 412 268 3398




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