Sequencing Gels Not Sticking to Whatmann

Kevin A. Morano kmorano at umich.edu
Fri Nov 7 09:51:06 EST 1997


Hi Rob,

   Sure, this can happen for a few reasons:

1. Your plate is not *really* clean.  Plates that don't look visibly dirty
can still cause problems.  Try a thorough wash with Liquinox in hot water,
followed by Windex and then two swipe-overs with 95% EtOH.  Plates should
be squeaky-clean.

2. You certainly can "sigmacoat" the other plate - just don't use too much
or your gel will creep up out from between the plates.  THe only problem
with this treatment is that you can't predict which plate the gel will
stick to, so you haveto be willing to flip the whole assembly over before
applying the Whatman.

3.   Try spritzing water on the paper before lowering it onto the gel,
then smooth over with your hands.  

Hope this helps.
Kevin

-- 
Kevin A. Morano
Dept. of Biological Chemistry
University of Michigan Medical School
Ann Arbor, MI 48109-0606



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