restriciton enzyme site

Ashok Aiyar aiyar at
Fri Nov 7 17:36:48 EST 1997

On 7 Nov 1997 07:02:12 GMT,
Marcel Tanudji (mtanudji at wrote:

>i have a question.  the restricion site for sma I is that of
>now if you have the sequence 
>would the enzyme still recognise it 

By "the enzyme" I presume you mean SmaI - no, it will not recognize
it, and it doesn't matter what topological form the substrate is in.

Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
aiyar at

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