restriciton enzyme site

[Ashok Aiyar]

On 7 Nov 1997 07:02:12 GMT,
Marcel Tanudji (mtanudji at cyllene.uwa.edu.au) wrote:

>i have a question.  the restricion site for sma I is that of
>
>5'cccggg3'
>3'gggccc5'
>
>now if you have the sequence 
>
>5'gggccc3'
>3'cccggg5'
>
>would the enzyme still recognise it 

By "the enzyme" I presume you mean SmaI - no, it will not recognize
it, and it doesn't matter what topological form the substrate is in.

Later,
Ashok
-- 
Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
aiyar at ebv.oncology.wisc.edu