restriciton enzyme site

[JP]

In article <63uedk$84u$1 at enyo.uwa.edu.au> mtanudji at cyllene.uwa.edu.au (Marcel Tanudji) writes:
In article <63uedk$84u$1 at enyo.uwa.edu.au> mtanudji at cyllene.uwa.edu.au (Marcel Tanudji) writes:
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>From: mtanudji at cyllene.uwa.edu.au (Marcel Tanudji)
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: restriciton enzyme site
>Date: 7 Nov 1997 07:02:12 GMT
>Organization: The University of Western Australia
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>hi all,

>i have a question.  the restricion site for sma I is that of

>5'cccggg3'
>3'gggccc5'

>now if you have the sequence 

>5'gggccc3'
>3'cccggg5'

>would the enzyme still recognise it and does it matter if its on a linear
>or circular DNA.

In my understanding, It will not recognise 5'gggccc3' and it does not matter 
if its on a linear or circular DNA.  Thinking about the structure of the DNA, 
circle does not change the 5' to 3' direction.  And the enzyme does not need 
an end to work.


>  -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
>  =  Marcel Tanudji                                                     =       
>  =  Dept Biochemistry,  University of Western Australia                =
>  =  Perth, Australia   [email:mtanudji at cyllene.uwa.edu.au]             =
>  =                                                                     =     
>  -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-